Phospho-rps6

Author: ntkb

August undefined, 2024

WebRPS6 is a 249 amino acid protein with a predicted molecular weight of 28.6 kD. Distribution Cytosol, nucleus, ER, ubiquitous expression. Function Phosphorylation events at a cluster … WebNational Center for Biotechnology Information

Purified anti-RPS6 Phospho (Ser244) Antibody - BioLegend

WebMar 9, 2024 · Flow Cytometry - Anti-RPS6 (phospho S235 + S236) antibody [SP50] (ab101691) Flow Cytometry analysis of PC-3 (Human prostate adenocarcinoma epithelial … WebJan 14, 2013 · S6 kinases (S6Ks) are mechanistic target of rapamycin substrates that participate in cell growth control. S6Ks phosphorylate ribosomal protein S6 (rpS6) and additional proteins involved in the ... deutsches partbred shetland pony

The mTOR-S6 kinase pathway promotes stress granule …

WebMar 9, 2024 · Anti-RPS6 (phospho S235 + S236) antibody [SP50] See all RPS6 primary antibodies Description Rabbit monoclonal [SP50] to RPS6 (phospho S235 + S236) Host species Rabbit Tested applications Suitable for: Dot blot, IHC-P, ICC/IF, Flow Cyt more details Species reactivity Reacts with: Mouse, Human Immunogen WebCells were then intracellularly stained with 2 µg/mL of purified anti-RPS6 Phospho (Ser244) antibody (clone A18024A) overnight at 4°C, after which proteins were visualized with Alexa Fluor® 594 goat anti-mouse IgG antibody (Cat. No. 405326) at 2.0 µg/mL. Nuclei were counterstained with DAPI, and the image was captured with a 60X objective. WebProduct name Anti-RPS6 (phospho S235) antibody [EP1338 (2)Y] - Ribosome Marker See all RPS6 primary antibodies Description Rabbit monoclonal [EP1338 (2)Y] to RPS6 (phospho S235) - Ribosome Marker Host species Rabbit Tested applications Suitable for: WB, IP, IHC-P, Dot blot more details Unsuitable for: Flow Cyt or ICC/IF Species reactivity deutsches theater göttingen theaterkasse

Combined quantification of intracellular (phospho …

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WebWestern blot analysis of Phospho-RPS6 (Ser240, Ser244) in untreated (–) and treated (+) 293T whole cell extract using Phospho-RPS6 (Ser240, Ser244) polyclonal antibody ( Product # PA5-85822) using 30 µg of sample at a dilution of 1:1000. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. WebAnimals were fasted overnight and refed ad lib for 4 h. Quantification of phospho-rpS6 is shown at the right, normalized to total rpS6. (*) P < 0.05 by Student's t-test. (D) Immunofluorescence staining of phospho-S6 (red) and TFE3 (green) in control (NTC) cells and cells lacking TSC1 or TSC2. Cells were starved or starved and restimulated with ... church emergency plans wordWebFeb 6, 2024 · RPS6 is phosphorylated downstream of active EGF signaling 13 and is lost within the first 24 hours of AG1478 treatment (Supplementary Fig. 4A). Quantification of … church emmanuel youtube

"WebCells were then intracellularly stained with 5 µg/mL (1:100 dilution) of Purified anti-RPS6 Phospho (Ser244) Antibody overnight at 4°C, after which proteins were visualized with Alexa Fluor® 594 Goat anti-mouse IgG Antibody (Cat. No. 405326) at 2.0 µg/mL. Nuclei were counterstained with DAPI, and the image was captured with a 60X objective. " - Phospho-rps6

Phospho-rps6

Regulation of Ribosomal Protein S6 Phosphorylation by Casein …

WebDec 21, 2024 · Ribosomal protein S6 (RPS6) is a component of the 40S small ribosomal subunit and participates in the control of mRNA translation. Additionally, phospho (p) … WebRPS6 Rabbit Polyclonal Antibody: 50μl: 593.00元: 加入购物车: AG3129: Phospho-S6 Ribosomal Protein (Ser235/236) Mouse Monoclonal Antibody: 50μl: 998.00元: 加入购物车: AG3133: Phospho-S6 Ribosomal Protein (Ser235/Ser236) Rabbit Monoclonal Antibody: 50μl: 998.00元: 加入购物车: AG3136: Phospho-S6 Ribosomal Protein (Ser240/244 ...

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WebPhospho-S6 Ribosomal Protein (Ser240/244) Antibody detects endogenous levels of ribosomal protein S6 only when phosphorylated at serines 240 and 244. This antibody does not detect S6 ribosomal protein phosphorylated … WebMar 22, 2024 · Both phosphorylation sites of rpS6 (S235/236 and S240/244) were detected with phospho-specific antibodies. Total protein levels of rpS6, FLAG and β-actin were also …

WebDot blot analysis of RPS6 (phospho S240 + S244) labeled with ab215214 at 1/1000 dilution. Lane 1: RPS6 (phospho S240 + S244) peptide. Lane 2: RPS6 (phospho S240) peptide. Lane 3: RPS6 (phospho S244) peptide. Lane 4: RPS6 non-phospho peptide. Goat Anti-Rabbit IgG H&L (HRP) at 1/100000 dilution was used as secondary antibody. WebRibosomal protein S6 (rpS6 or eS6) is a component of the 40S ribosomal subunit and is therefore involved in translation. Mouse model studies have shown that phosphorylation …

WebRPS6 Antibodies Target Information Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. WebResults: The activity of a downstream effector of the HER2 pathway phosphorylated ribosomal protein S6 (p-rpS6), was suppressed by trastuzumab in the parental cell lines …

WebThe phosphorylation of ribosomal protein S6 (rpS6) has been described for the first time about four decades ago. Since then, numerous studies have shown that this modification …

WebMar 9, 2024 · Levels of phosphorylated RPS6 (p-RPS6) and S6 kinases (p-S6K) are shown to indicate the effectiveness of rapamycin at inhibiting mTORC1 activity. church emergency plans pdfWebFeb 6, 2024 · We developed single-cell RNA and Immunodetection (RAID) to allow combined analysis of the transcriptome and intracellular (phospho-)proteins from fixed single cells. RAID successfully... deutsches theater programmWebApr 10, 2024 · Purpose: To present a case of a chemotherapy regimen combining a fibroblast growth factor receptor (FGFR) and mitogen-activated protein kinase kinase … deutsches public health institutWeb40S Ribosomal Protein S6 (RPS6) is the major substrate of protein kinases, particularly p70 S6 kinase, in eukaryotic ribosomes. RPS6 phosphorylation at S235, S236, S240, and S244 upregulates the translation of mRNAs containing an oligopyrimidine tract at their transcriptional start sites. This phosphorylation is stimulated by growth factors ... church emergency response planWebImmunohistochemical analysis of Phospho-RPS6 pSer240 in paraffin-embedded human breast carcinoma using Phospho-RPS6 pSer240 polyclonal antibody (Product # PA5-36759) at a dilution of 1:100. Western blot analysis of Phospho-S6 (Ser240) in Lane 1: HeLa whole cell lysate (40 µg), Lane 2: HeLa treated with serum starved (24h) whole cell lysate (40 ... deutsche structured finance gmbhWebPhospho-S6 (Ser240, Ser244) Antibody (701845) in ICC/IF. For immunofluorescence analysis HEK-293 cells were fixed and permeabilized for detection of RPS6 (pS240/244) using RPS6 (pS240/244) Recombinant Rabbit Monoclonal Antibody ( Product # 701845, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, … deutsches theater münchen dirty dancingWebWB analysis using 80206-1-RR. Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80206-1-RR (Phospho-S6 Ribosomal protein (Ser236) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. church employee income for se tax