WebTheis (1935) obtained a solution of this equation based on an analogy between groundwater flow towards a well and flow of heat towards a sink or point where heat is removed at a uniform rate just as water is removed from the well at a constant rate. Thus by assuming that the well is replaced by a mathematical sink of constant strength and ... WebAdd 10 μl of the CCK-8 solution to each well of the plate. Note: Be careful not to introduce bubbles to the wells, since they interfere with the O.D. reading. 4) Incubate the plate for 1-4 hours in the incubator. 5) Measure the absorbance at 450 nm using a microplate reader. Prepare a calibration curve using the
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